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Proceedings Paper

Coherent control in multiphoton fluorescence imaging
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Paper Abstract

In multiphoton fluorescence laser-scanning microscopy ultrafast laser pulses, i.e. light pulses having pulse-width ≤ 1picosecond (1 ps = 10-12 s), are commonly used to circumvent the low multiphoton absorption cross-sections of common fluorophores. Starting with a discussion on how amplitude modulation of ultrashort pulse-train enhances the two-photon fluorescence providing deep insight into laser-induced photo-thermal damage, the effect of controlling time lag between phase-locked laser pulses on imaging is described. In addition, the prospects of laser pulse-shaping in signal enhancement (by temporal pulse-compression at the sample) and selective excitation of fluorophores (by manipulating the phase and/or amplitude of different frequency components within the pulse) are discussed with promising future applications lying ahead.

Paper Details

Date Published: 25 February 2009
PDF: 6 pages
Proc. SPIE 7183, Multiphoton Microscopy in the Biomedical Sciences IX, 71832B (25 February 2009); doi: 10.1117/12.807687
Show Author Affiliations
Arijit Kumar De, Indian Institute of Technology Kanpur (India)
Debabrata Goswami, Indian Institute of Technology Kanpur (India)

Published in SPIE Proceedings Vol. 7183:
Multiphoton Microscopy in the Biomedical Sciences IX
Ammasi Periasamy; Peter T. C. So, Editor(s)

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