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Proceedings Paper

High volume confinement in two-photon fluorescence correlation spectroscopy with radially polarized light
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Paper Abstract

We present the results on two-photon total-internal-reflection fluorescence correlation spectroscopy. The combination of liquid crystal spatial light modulator, providing radial polarization, with ultrafast laser (picosecond Nd:GdVO4 laser) allowed us to take the advantage of nonlinear optical contrast mechanisms to suppress the side-lobe energy specific for radial polarization and reduce the effective excited volume twice compared to one-photon evanescent wave excitation in fluorescence correlation spectroscopy.

Paper Details

Date Published: 25 February 2009
PDF: 6 pages
Proc. SPIE 7185, Single Molecule Spectroscopy and Imaging II, 71850D (25 February 2009); doi: 10.1117/12.807596
Show Author Affiliations
D. Ivanov, Lab. d'Optique Biomedicale, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
V. Shcheslavskiy, Lab. d'Optique Biomedicale, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
I. Märki, Lab. d'Optique Biomedicale, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
M. Leutenegger, Max-Planck-Institut für Biophysikalische Chemie (Germany)
T. Lasser, Lab. d'Optique Biomedicale, Ecole Polytechnique Fédérale de Lausanne (Switzerland)


Published in SPIE Proceedings Vol. 7185:
Single Molecule Spectroscopy and Imaging II
Jörg Enderlein; Zygmunt Karol Gryczynski; Rainer Erdmann, Editor(s)

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