Share Email Print

Proceedings Paper

Evaluation of membrane stacking in electron microscope images
Author(s): Gilles Hermann; Argyro Karathanou; Jean-Luc Buessler; Jean-Philippe Urban
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

The objective of our work is to develop a tool for automatic analysis of 2D membrane protein crystal images in Transmission Electron Microscopy (TEM). The success of crystallization experiments is evaluated at high magnification. The crystalline structure of a membrane can be observed when no other membranes are superposed. It is therefore necessary to identify mono-layer membranes. In this paper we introduce an algorithm that determines the stacking-level of membranes. Our method determines a quantum, a gray-level quantity that is characteristic of a non-stacked membrane. In this way we are able to label each region qualitatively and construct a stacking-level map that distinguishes from non-stacked to up to four-level stacked membranes. This map provides the regions that will trigger a new image acquisition at higher magnification.

Paper Details

Date Published: 3 February 2009
PDF: 11 pages
Proc. SPIE 7251, Image Processing: Machine Vision Applications II, 725110 (3 February 2009); doi: 10.1117/12.807268
Show Author Affiliations
Gilles Hermann, Univ. de Haute Alsace (France)
Argyro Karathanou, Univ. de Haute Alsace (France)
Jean-Luc Buessler, Univ. de Haute Alsace (France)
Jean-Philippe Urban, Univ. de Haute Alsace (France)

Published in SPIE Proceedings Vol. 7251:
Image Processing: Machine Vision Applications II
Kurt S. Niel; David Fofi, Editor(s)

© SPIE. Terms of Use
Back to Top