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Proceedings Paper

Micro amperometric immunosensor for the detection of salmonella typhimurium
Author(s): Jizhou Sun; Shanhong Xia; Chao Bian; Lan Qu
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Paper Abstract

In this paper, a micro amperometric immunosensor based on Micro-Electro-Mechanical Systems technology for the detection of Salmonella typhimurium (S. typhimurium) was constructed by immobilizing a polyclonal antibody (the bio-molecular recognition element) onto the surface of polypyrrole(PPy) /staphylococcal protein A(SPA) modified Pt electrode. Pyrrole doped with SPA was co-electropolymerized onto the working electrode surface by cyclic voltammetry in 10 minutes for orientation-controlled immobilization of salmonella capture antibodies. S. typhimurium with the concentration of 102cfu/ml could be detected by this immunosensor with a controllable and convenient manipulation to effectively modify the sensing surface more rapidly with less consumption of reagent (10µL), which showed the good property of the sensor. It is potential to develop a micro biosensor that can be used for convenient, accurate, cost-effective and real-time sensing of pathogens in food products.

Paper Details

Date Published: 3 February 2009
PDF: 6 pages
Proc. SPIE 7159, 2008 International Conference on Optical Instruments and Technology: MEMS/NEMS Technology and Applications, 71590A (3 February 2009); doi: 10.1117/12.807108
Show Author Affiliations
Jizhou Sun, Institute of Electronics (China)
Graduate Univ. of the Chinese Academy of Sciences (China)
Shanhong Xia, Institute of Electronics (China)
Chao Bian, Institute of Electronics (China)
Lan Qu, Institute of Electronics (China)
Graduate Univ. of the Chinese Academy of Sciences (China)


Published in SPIE Proceedings Vol. 7159:
2008 International Conference on Optical Instruments and Technology: MEMS/NEMS Technology and Applications

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