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Proceedings Paper

Design of time-resolved fluorometer based on immunochromatography
Author(s): Bingqiang Ren; Lihua Huang; Huijie Huang
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Paper Abstract

This paper introduces the design of a novel time-resolved fluorometer based on immunochromatograghy. Different from the other time-resolved fluorometers, it tests the immunochromatographic strip which is labeled with lanthanide ions and their chelates. This instrument can provide a rapid, quantitative measurement of analytes present in samples without any washing steps and it can be used to carry out point-of-care test (POCT). The immunochromatograghy-based timeresolved fluorometer is composed of a specific optical sensor, a scanning stage, a signal processing system and a computer control system. The light from UV LED is focused on the test strip by a condense lens group in the optical sensor. If the labels are present in samples, the fluorescence at 613nm will be exited (when Eu3+chelate is used for marking substance). After a delay of some microseconds, the fluorescence will be collected by the optical sensor and converted into electronic signal by a photomultiplier tube (PMT). The concentration of the sample can be calculated through the standard working curve of this instrument. By testing, the sensitivity is several ng/ml level (when Eu3+chelate is used for marking substance), test linear range is from several ng/ml to 103 ng/ml, in which correlation coefficient is 99.97%.

Paper Details

Date Published: 3 February 2009
PDF: 7 pages
Proc. SPIE 7157, 2008 International Conference on Optical Instruments and Technology: Advanced Sensor Technologies and Applications, 71570Y (3 February 2009); doi: 10.1117/12.806776
Show Author Affiliations
Bingqiang Ren, Shanghai Institute of Optics and Fine Mechanics (China)
Graduate Univ. of the Chinese Academy of Sciences (China)
Lihua Huang, Shanghai Institute of Optics and Fine Mechanics (China)
Graduate Univ. of the Chinese Academy of Sciences (China)
Huijie Huang, Shanghai Institute of Optics and Fine Mechanics (China)
Graduate Univ. of the Chinese Academy of Sciences (China)


Published in SPIE Proceedings Vol. 7157:
2008 International Conference on Optical Instruments and Technology: Advanced Sensor Technologies and Applications

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