Share Email Print
cover

Proceedings Paper

Obstacles to the production of protein microarray cassettes
Author(s): Jean Montagu; Herman DeWeerd; Nathan Tyburczy; Natalia Rodionova; Peter Maimonis
Format Member Price Non-Member Price
PDF $14.40 $18.00

Paper Abstract

This paper examines the necessary technologies to be mastered in order to build a practical micro array-based immunoassay cassette and its processing station for protein analysis. The interdependence of surface-chemistry, dye stability and imaging are outlined showning why a treated 100 nm film of Nitrocellulose adhered by an intervening layer to glass offers an efficacious surface for immobilizing an array of protein probes. The properties of a storage surface to support in desiccated form, fluidize and transport additional reagents are outlined and a practical solution proposed. Wet and Dry imaging are compared. The steps and functions expected for an assay platform comprising processing station and biochip cassette are identified. The performance of a successful bench-top automated multiplex immunoassay system is briefly described

Paper Details

Date Published: 24 February 2009
PDF: 9 pages
Proc. SPIE 7207, Microfluidics, BioMEMS, and Medical Microsystems VII, 72070F (24 February 2009); doi: 10.1117/12.806639
Show Author Affiliations
Jean Montagu, Decision BioMarkers Inc. (United States)
Herman DeWeerd, Decision BioMarkers Inc. (United States)
Nathan Tyburczy, Decision BioMarkers Inc. (United States)
Natalia Rodionova, Decision BioMarkers Inc. (United States)
Peter Maimonis, Decision BioMarkers Inc. (United States)


Published in SPIE Proceedings Vol. 7207:
Microfluidics, BioMEMS, and Medical Microsystems VII
Wanjun Wang, Editor(s)

© SPIE. Terms of Use
Back to Top