Share Email Print
cover

Proceedings Paper

Bacterial mixture analysis with Raman chemical imaging microspectroscopy
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

Raman chemical imaging microspectroscopy (RCIM) is being evaluated as a technology for waterborne pathogen detection. Binary and ternary mixtures including combinations of polystyrene beads, Grampositive Bacillus anthracis and B. atrophaeus spores, B. cereus vegetative cells, and Gram-negative E. coli cells were investigated by RCIM for differentiation and characterization purposes. We have demonstrated the ability of RCIM, in combination with Pearson's cross correlation and multivariate principal components analysis data reduction techniques, to differentiate these components in the same field of view (FOV). Conventional applications of RCIM consist of differentiating relatively broad areas in a FOV. Here, RCIM is expanded in its capabilities to differentiate and distinguish between different micron size species in single particles and clusters of mixed species.

Paper Details

Date Published: 8 May 2009
PDF: 10 pages
Proc. SPIE 7304, Chemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing X, 730403 (8 May 2009); doi: 10.1117/12.806456
Show Author Affiliations
Ashish Tripathi, Science Applications International Corp. (United States)
Rabih E. Jabbour, Science Applications International Corp. (United States)
Jason A. Guicheteau, U.S. Army Edgewood Chemical Biological Ctr. (United States)
Steven D. Christesen, U.S. Army Edgewood Chemical Biological Ctr. (United States)
Darren K. Emge, U.S. Army Edgewood Chemical Biological Ctr. (United States)
Janet L. Jensen, U.S. Army Edgewood Chemical Biological Ctr. (United States)
A. Peter Snyder, U.S. Army Edgewood Chemical Biological Ctr. (United States)


Published in SPIE Proceedings Vol. 7304:
Chemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing X
Augustus W. Fountain; Patrick J. Gardner, Editor(s)

© SPIE. Terms of Use
Back to Top