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Proceedings Paper

Detection of mitochondrial DNA with the compact bead array sensor system (cBASS)
Author(s): Shawn P. Mulvaney; Carol N. Ibe; Jane M. Caldwell; Jay F. Levine; Lloyd J. Whitman; Cy R. Tamanaha
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Paper Abstract

Enteric pathogens are a significant contaminant in surface waters used for recreation, fish and shellfish harvesting, crop irrigation, and human consumption. The need for water monitoring becomes more pronounced when industrial, agricultural, and residential lands are found in close proximity. Fecal contamination is particularly problematic and identification of the pollution source essential to remediation efforts. Standard monitoring for fecal contamination relies on indicator organisms, but the technique is too broad to identify the source of contamination. Instead, real-time PCR of mitochondrial DNA (mtDNA) is an emerging method for identification of the contamination source. Presented herein, we evaluate an alternative technology, the compact Bead Array Sensor System (cBASS®) and its assay approach Fluidic Force Discrimination (FFD), for the detection of mtDNA. Previously, we achieved multiplexed, attomolar detection of toxins and femtomolar detection of nucleic acids in minutes with FFD assays. More importantly, FFD assays are compatible with a variety of complex matrices and therefore potentially applicable for samples where the matrix would interfere with PCR amplification. We have designed a triplex assay for the NADH gene found in human, swine, and bovine mtDNA and demonstrated the specific detection of human mtDNA spiked into a waste water sample.

Paper Details

Date Published: 19 February 2009
PDF: 10 pages
Proc. SPIE 7167, Frontiers in Pathogen Detection: From Nanosensors to Systems, 71670V (19 February 2009); doi: 10.1117/12.806433
Show Author Affiliations
Shawn P. Mulvaney, Naval Research Lab. (United States)
Carol N. Ibe, Naval Research Lab. (United States)
Jane M. Caldwell, North Carolina State Univ. (United States)
Jay F. Levine, North Carolina State Univ. (United States)
Lloyd J. Whitman, Naval Research Lab. (United States)
Cy R. Tamanaha, Naval Research Lab. (United States)

Published in SPIE Proceedings Vol. 7167:
Frontiers in Pathogen Detection: From Nanosensors to Systems
Philippe M. Fauchet, Editor(s)

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