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Proceedings Paper

Compression of multispectral fluorescence microscopic images based on a modified set partitioning in hierarchal trees
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Paper Abstract

Modern automated microscopic imaging techniques such as high-content screening (HCS), high-throughput screening, 4D imaging, and multispectral imaging are capable of producing hundreds to thousands of images per experiment. For quick retrieval, fast transmission, and storage economy, these images should be saved in a compressed format. A considerable number of techniques based on interband and intraband redundancies of multispectral images have been proposed in the literature for the compression of multispectral and 3D temporal data. However, these works have been carried out mostly in the elds of remote sensing and video processing. Compression for multispectral optical microscopy imaging, with its own set of specialized requirements, has remained under-investigated. Digital photography{oriented 2D compression techniques like JPEG (ISO/IEC IS 10918-1) and JPEG2000 (ISO/IEC 15444-1) are generally adopted for multispectral images which optimize visual quality but do not necessarily preserve the integrity of scientic data, not to mention the suboptimal performance of 2D compression techniques in compressing 3D images. Herein we report our work on a new low bit-rate wavelet-based compression scheme for multispectral fluorescence biological imaging. The sparsity of signicant coefficients in high-frequency subbands of multispectral microscopic images is found to be much greater than in natural images; therefore a quad-tree concept such as Said et al.'s SPIHT1 along with correlation of insignicant wavelet coefficients has been proposed to further exploit redundancy at high-frequency subbands. Our work propose a 3D extension to SPIHT, incorporating a new hierarchal inter- and intra-spectral relationship amongst the coefficients of 3D wavelet-decomposed image. The new relationship, apart from adopting the parent-child relationship of classical SPIHT, also brought forth the conditional "sibling" relationship by relating only the insignicant wavelet coefficients of subbands at the same level of decomposition. The insignicant quadtrees in dierent subbands in the high-frequency subband class are coded by a combined function to reduce redundancy. A number of experiments conducted on microscopic multispectral images have shown promising results for the proposed method over current state-of-the-art image-compression techniques.

Paper Details

Date Published: 10 February 2009
PDF: 11 pages
Proc. SPIE 7245, Image Processing: Algorithms and Systems VII, 72450J (10 February 2009); doi: 10.1117/12.806038
Show Author Affiliations
Awais Mansoor, Purdue Univ. (United States)
J. Paul Robinson, Purdue Univ. (United States)
Bartek Rajwa, Purdue Univ. (United States)

Published in SPIE Proceedings Vol. 7245:
Image Processing: Algorithms and Systems VII
Nasser M. Nasrabadi; Jaakko T. Astola; Karen O. Egiazarian; Syed A. Rizvi, Editor(s)

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