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Proceedings Paper

Super-resolution imaging combining the design of an optical coherence microscope objective with liquid-lens based dynamic focusing capability and computational methods
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Paper Abstract

In this paper, we present the design of a 0.2 NA microscope objective operating across a 120nm broadband spectral range that requires only two doublets and an embedded liquid lens to achieve 3 μm invariant lateral resolution throughout a large 8 cubic millimeter imaging sample. Achieving invariant lateral resolution comes with some sacrifice in imaging speed, yet in the approach proposed, high speed in vivo imaging is maintained up to a resolution of 3 μm for a 2x2 mm sample size. Thus, in anticipation to ultimately aim for a resolution of 0.5 to 1 μm, we are investigating the possibility to further gain in resolution using super-resolution methods so both hardware solutions and image processing methods together can provide the best trade-off in overall resolution and speed of imaging. As a starting point to investigate super-resolution methods, we evaluate in this paper three well-known super-resolution algorithms used to reconstruct a high resolution image from down-sampled low resolution images of an African frog tadpole acquired en face using our OCM set-up. To establish ground truth necessary for assessment of the methods, low resolution images were simulated from a high resolution OCM image. The specification and design performance of the custom designed microscope will be presented as well as our first results of super-resolution imaging. The performance of each algorithm was analyzed and all performances compared using two different metrics. Early results indicate that super-resolution may play a significant role in the optimization of high invariant resolution OCM systems.

Paper Details

Date Published: 11 September 2008
PDF: 11 pages
Proc. SPIE 7061, Novel Optical Systems Design and Optimization XI, 70610C (11 September 2008); doi: 10.1117/12.805365
Show Author Affiliations
Apurva Jain, College of Optics and Photonics, Univ. of Central Florida (United States)
Supraja Murali, College of Optics and Photonics, Univ. of Central Florida (United States)
Nicolene Papp, College of Optics and Photonics, Univ. of Central Florida (United States)
Kevin Thompson, Optical Research Associates (United States)
Kye-sung Lee, College of Optics and Photonics, Univ. of Central Florida (United States)
Panomsak Meemon, College of Optics and Photonics, Univ. of Central Florida (United States)
Hassan Foroosh, Univ. of Central Florida (United States)
Jannick P. Rolland, College of Optics and Photonics, Univ. of Central Florida (United States)
Univ. of Central Florida (United States)


Published in SPIE Proceedings Vol. 7061:
Novel Optical Systems Design and Optimization XI
R. John Koshel; G. Groot Gregory; James D. Moore; David H. Krevor, Editor(s)

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