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Proceedings Paper

Application of laser to measurement of cyclic contractile movement of cultured myotubes
Author(s): Shigehiro Hashimoto; Hiroyuki Tonami; Eiji Yamada; Shuichi Mochizuki; Jun Takase; Mieko Ohsuga
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Paper Abstract

Laser technique has been applied to measure cyclic contractile movement of cultured myotubes in vitro. The designed measurement system includes light source (helium neon, 632.8 nm wave length), charge-coupled devise cameras and detectors. Cyclic contraction of myotubes cultured from C2C12 (mouse myoblast) was generated by cyclic electric pulses (amplitude < 60 V, 1 ms width) through electrodes of platinum wire dipped in the medium. The spectrum of fluctuating intensity of the transmitted laser beam through the myotubes, which periodically repeated contraction and relaxation, was analyzed. The results show that the designed laser system is effective to detect frequency of cyclic contractile movement of myotubes between 0.5 Hz and 5 Hz.

Paper Details

Date Published: 12 February 2009
PDF: 8 pages
Proc. SPIE 7179, Optics in Tissue Engineering and Regenerative Medicine III, 71790D (12 February 2009); doi: 10.1117/12.805234
Show Author Affiliations
Shigehiro Hashimoto, Osaka Institute of Technology (Japan)
Hiroyuki Tonami, Kyoto Univ. (Japan)
Eiji Yamada, Osaka Institute of Technology (Japan)
Shuichi Mochizuki, Osaka Institute of Technology (Japan)
Jun Takase, Osaka Institute of Technology (Japan)
Mieko Ohsuga, Osaka Institute of Technology (Japan)

Published in SPIE Proceedings Vol. 7179:
Optics in Tissue Engineering and Regenerative Medicine III
Sean J. Kirkpatrick; Ruikang Wang, Editor(s)

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