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Proceedings Paper

A simple mixture to enhance muscle transmittance
Author(s): Luís Oliveira; Armindo Lage; Manuel Pais Clemente; Valery V. Tuchin
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Paper Abstract

Skeletal muscle is a fibrous tissue composed by muscle fibers and interstitial fluid. Due to this constitution, the muscle presents a non uniform refractive index profile that origins strong light scattering. One way to improve tissue transmittance is to reduce this refractive index mismatch by immersing the muscle in an optical clearing agent. As a consequence of such immersion tissue also suffers dehydration. The study of the optical clearing effect created by a simple mixture composed by ethanol, glycerol and distilled water has proven its effectiveness according to the variations observed in the parameters under study. The effect was characterized in terms of its magnitude, time duration and histological variations. The applied treatment has created a small reduction of the global sample refractive index that is justified by the long time rehydration caused by water in the immersing solution. From the reduction in sample pH we could also identify the dehydration process created in the sample. The immersion treatment has originated fiber bundle contraction and a spread distribution of the muscle fiber bundles inside. New studies with the mixture used, or with other combinations of its constituents might be interesting to perform with the objective to develop new clinical procedures.

Paper Details

Date Published: 9 June 2008
PDF: 11 pages
Proc. SPIE 6791, Saratov Fall Meeting 2007: Optical Technologies in Biophysics and Medicine IX, 67910J (9 June 2008); doi: 10.1117/12.803978
Show Author Affiliations
Luís Oliveira, Ctr. de Ciências e Tecnologias Ópticas (Portugal)
Armindo Lage, Porto Univ. (Portugal)
Manuel Pais Clemente, Ctr. de Ciências e Tecnologias Ópticas (Portugal)
Valery V. Tuchin, Saratov State Univ. (Russia)


Published in SPIE Proceedings Vol. 6791:
Saratov Fall Meeting 2007: Optical Technologies in Biophysics and Medicine IX

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