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Proceedings Paper

Cutaneous tumors in vivo investigations using fluorescence and diffuse reflectance techniques
Author(s): E. Borisova; P. Troyanova; E. Nikolova; L. Avramov
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Paper Abstract

In the recent years, there has been growing interest in the common use of laser-induced autofluorescence (LIAF) and reflectance spectroscopy (RS) to differentiate disease from normal surrounding tissue - so called optical biopsy method. Painless, instant diagnoses from optical biopsies will soon be a reality. These forms of optical diagnoses are preferable to the removal of several square millimeters of tissue surface - common in traditional biopsies - followed by delays while samples are sent for clinical analysis. The goal of this work was investigation of cutaneous benign and malignant lesions by the methods of LIAFS and RS. A nitrogen laser at 337 nm was applied for the needs of autofluorescence excitation. Broad-spectrum halogen lamp (from 400 to 900 nm) was applied for diffuse reflectance measurements. An associated microspectrometer detected in vivo the fluorescence and reflectance signals from human skin. The main spectral features of benign lesions - compound nevus, dysplastic nevi, heamangioma and basal cell papilloma and malignant lesions - pigmented, amelanotic and secondary malignant melanoma, as well as basal cell carcinoma are discussed and their possible origins are indicated. Spectra from healthy skin areas near to the lesion were detected to be used posteriori to reveal changes between healthy and lesion skin spectra. Influence of the main skin pigments on the spectra detected is discussed and evaluation of possibilities for differentiation between malignant and benign lesions is made based on their spectral properties. This research shows that non-invasive and high-sensitive in vivo detection by means of appropriate light sources and detectors should be possible, related to real-time determination of existing pathological conditions.

Paper Details

Date Published: 9 June 2008
PDF: 10 pages
Proc. SPIE 6791, Saratov Fall Meeting 2007: Optical Technologies in Biophysics and Medicine IX, 679105 (9 June 2008); doi: 10.1117/12.803963
Show Author Affiliations
E. Borisova, Institute of Electronics (Bulgaria)
P. Troyanova, National Oncological Diagnostic Ctr. (Bulgaria)
E. Nikolova, National Oncological Diagnostic Ctr. (Bulgaria)
L. Avramov, Institute of Electronics (Bulgaria)

Published in SPIE Proceedings Vol. 6791:
Saratov Fall Meeting 2007: Optical Technologies in Biophysics and Medicine IX

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