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Proceedings Paper

Digital holographic microscope with low spatial and temporal coherence of illumination
Author(s): Hana Janečková; Pavel Kolman; Pavel Veselý; Radim Chmelík
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Paper Abstract

In this paper we present a newly developed digital transmission holographic microscope. The microscope enables using arbitrarily low coherent illumination (both spatially and temporally) in conjunction with the off-axis holography. The setup of the microscope, its function and the object wave reconstruction procedure are described. The optical sectioning effect, similar to a confocal microscope, resulting from the use of low spatially coherent light source is demonstrated. The microscope has been tailored for studies of living cell dynamics. Time-lapse phase reconstruction series of live cells activities were carried out. The different behavior related to changes in the cell cycle is demonstrated.

Paper Details

Date Published: 25 April 2008
PDF: 8 pages
Proc. SPIE 7000, Optical and Digital Image Processing, 70002E (25 April 2008); doi: 10.1117/12.800392
Show Author Affiliations
Hana Janečková, Brno Univ. of Technology (Czech Republic)
Pavel Kolman, Brno Univ. of Technology (Czech Republic)
Pavel Veselý, Institute of Molecular Genetics AS CR (Czech Republic)
Radim Chmelík, Brno Univ. of Technology (Czech Republic)

Published in SPIE Proceedings Vol. 7000:
Optical and Digital Image Processing
Peter Schelkens; Touradj Ebrahimi; Gabriel Cristóbal; Frédéric Truchetet, Editor(s)

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