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Proceedings Paper

Electrokinetic delivery of single fluorescent biomolecules in fluidic nanochannels
Author(s): Lloyd M. Davis; Brian K. Canfield; Xiaoxuan Li; William H. Hofmeister; Guoqing Shen; Isaac P. Lescano-Mendoza; Bruce W. Bomar; John P. Wikswo; Dmitry A. Markov; Philip C. Samson; Claus Daniel; Zbigniew Sikorski; William N. Robinson
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Paper Abstract

We describe the fabrication of sub-100-nanometer-sized channels in a fused silica lab-on-a-chip device and experiments that demonstrate detection of single fluorescently labeled proteins in buffer solution within the device with high signal and low background. The fluorescent biomolecules are transported along the length of the nanochannels by electrophoresis and/or electro-osmosis until they pass into a two-focus laser irradiation zone. Pulse-interleaved excitation and time-resolved single-photon detection with maximum-likelihood analysis enables the location of the biomolecule to be determined. Diffusional transport of the molecules is found to be slowed within the nanochannel, and this facilitates fluidic trapping and/or prolonged measurements on individual biomolecules. Our goal is to actively control the fluidic transport to achieve rapid delivery of each new biomolecule to the sensing zone, following the completion of measurements, or the photobleaching of the prior molecule. We have used computer simulations that include photophysical effects such as triplet crossing and photobleaching of the labels to design control algorithms, which are being implemented in a custom field-programmable-gate-array circuit for the active fluidic control.

Paper Details

Date Published: 29 August 2008
PDF: 12 pages
Proc. SPIE 7035, Biosensing, 70350A (29 August 2008); doi: 10.1117/12.798173
Show Author Affiliations
Lloyd M. Davis, Univ. of Tennessee Space Institute (United States)
Brian K. Canfield, Univ. of Tennessee Space Institute (United States)
Xiaoxuan Li, Univ. of Tennessee Space Institute (United States)
William H. Hofmeister, Univ. of Tennessee Space Institute (United States)
Guoqing Shen, Univ. of Tennessee Space Institute (United States)
Isaac P. Lescano-Mendoza, Univ. of Tennessee Space Institute (United States)
Bruce W. Bomar, Univ. of Tennessee Space Institute (United States)
John P. Wikswo, Vanderbilt Univ. (United States)
Dmitry A. Markov, Vanderbilt Univ. (United States)
Philip C. Samson, Vanderbilt Univ. (United States)
Claus Daniel, Oak Ridge National Lab. (United States)
Zbigniew Sikorski, Univ. of Tennessee Space Institute (United States)
William N. Robinson, Univ. of Tennessee Space Institute (United States)


Published in SPIE Proceedings Vol. 7035:
Biosensing
Manijeh Razeghi; Hooman Mohseni, Editor(s)

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