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Proceedings Paper

Bioadhesive nanoareas in antifouling matrix for highly efficient affinity sensors
Author(s): I. Mannelli; A. Valsesia; P. Lisboa; M. Anger-Leroy; S. Mornet; P. Colpo; F. Rossi
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Paper Abstract

A procedure for fabricating nanopatterned surfaces at the sub-500 nm scale comprising a hexagonal close packed array of bioadhesive gold nanoareas in a protein resistant matrix (PEO-like polymer), has been optimized. The surfaces were characterized by AFM analysis and their interaction with amino functionalised gold nanoparticles as models were investigated. The AFM images show the crystalline arrangement of nanopattern array and the localized adsorption of the H2N-Au nanoparticles in the bioadhesive nanoareas. A Surface Plasmon Resonance imaging (SPRi) system was used to assess the detection performances of these surfaces when employed as a transduction platform for studying biomolecule interactions. The investigated surfaces showed an enhancement of the affinity reaction efficiency with respect to the non structured surfaces. The obtained preliminary results show that nanostructuring the surfaces improve the binding site accessibility of the immobilized biological probes without significantly modifying the native biomolecule conformation.

Paper Details

Date Published: 29 August 2008
PDF: 10 pages
Proc. SPIE 7035, Biosensing, 70350Y (29 August 2008); doi: 10.1117/12.796969
Show Author Affiliations
I. Mannelli, Institute for Health and Consumer Protection (Italy)
A. Valsesia, Institute for Health and Consumer Protection (Italy)
P. Lisboa, Institute for Health and Consumer Protection (Italy)
M. Anger-Leroy, GenOptics SA (France)
S. Mornet, Condensed Matter Chemistry Institute of Bordeaux (France)
P. Colpo, Institute for Health and Consumer Protection (Italy)
F. Rossi, Institute for Health and Consumer Protection (Italy)

Published in SPIE Proceedings Vol. 7035:
Manijeh Razeghi; Hooman Mohseni, Editor(s)

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