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Proceedings Paper

Single and double stranded DNA detection using locked nucleic acid (LNA) functionalized nanoparticles
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Paper Abstract

Gold and silver nanoparticles functionalized with oligonucleotides can be used for the detection of specific sequences of DNA. We show that gold nanoparticles modified with locked nucleic acid (LNA) form stronger duplexes with a single stranded DNA target and offer better discrimination against single base pair mismatches than analogous DNA probes. Our LNA nanoparticle probes have also been used to detect double stranded DNA through triplex formation, whilst still maintaining selectivity for only complementary targets. Nanoparticle conjugates embedded with suitable surface enhanced resonance Raman scattering (SERRS) labels have been synthesized enabling simultaneous detection and identification of multiple DNA targets.

Paper Details

Date Published: 29 August 2008
PDF: 10 pages
Proc. SPIE 7035, Biosensing, 70350J (29 August 2008); doi: 10.1117/12.794965
Show Author Affiliations
Fiona McKenzie, Univ. of Strathclyde (United Kingdom)
Robert Stokes, Univ. of Strathclyde (United Kingdom)
Karen Faulds, Univ. of Strathclyde (United Kingdom)
Duncan Graham, Univ. of Strathclyde (United Kingdom)

Published in SPIE Proceedings Vol. 7035:
Manijeh Razeghi; Hooman Mohseni, Editor(s)

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