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Proceedings Paper

Compensation method for quantitative observation of multicolor fluorescence with nonlinear mapping
Author(s): Soichiro Morishita; Hideo Yokota; Hajime Asama; Ryutaro Himeno; Taketoshi Mishima
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Paper Abstract

In the field of biology, compensation of multiple fluorescence is necessary for quantitative measurement of samples. Conventional methods depend on a linear combination model irrespective of the model's adequacy. Therefore, proper compensation has not been performed in some situations. To overcome this problem, we propose a method for performing nonlinear mapping with emphasis on the distribution of samples on a plane of which the base vectors are references of fluorescence. This paper describes an experiment with measurement of multiple fluorescence and compensation using a conventional method and our proposed method. Results show that multiple fluorescence is not always able to assume a linear combination model. Moreover, we confirmed that the presented method is independent of linearity of multiple fluorescence.

Paper Details

Date Published: 5 September 2008
PDF: 10 pages
Proc. SPIE 7075, Mathematics of Data/Image Pattern Recognition, Compression, and Encryption with Applications XI, 70750J (5 September 2008); doi: 10.1117/12.794348
Show Author Affiliations
Soichiro Morishita, Univ. of Tokyo (Japan)
Hideo Yokota, RIKEN (Japan)
Hajime Asama, Univ. of Tokyo (Japan)
Ryutaro Himeno, RIKEN (Japan)
Taketoshi Mishima, Saitama Univ. (Japan)


Published in SPIE Proceedings Vol. 7075:
Mathematics of Data/Image Pattern Recognition, Compression, and Encryption with Applications XI
Mark S. Schmalz; Gerhard X. Ritter; Junior Barrera; Jaakko T. Astola, Editor(s)

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