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Proceedings Paper

How to measure slow diffusion in yeast cell membranes
Author(s): Jonas Ries; Christian Klose; Christiane Walch-Solimena; Petra Schwille
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Paper Abstract

Here we present two complementary methods for accurate diffusion measurements in yeast cell membranes. Fluorescence spreading after photobleaching analyzes the blurring of an initially sharp border between bleached and unbleached parts of the membrane. Two-focus scanning fluorescence correlation spectroscopy requires only a low concentration of labeled fluorophores and allows for very long measurement times due to correction for instabilities necessary to probe the slow diffusion in yeast plasma membranes. We apply these techniques to study the dynamics of different transmembrane proteins in the plasma membrane of the yeast Saccharomyces cerevisiae. The differences in the diffusion coefficients support the idea of co-existing membrane microdomains in the yeast plasma membrane.

Paper Details

Date Published: 2 May 2008
PDF: 8 pages
Proc. SPIE 6991, Biophotonics: Photonic Solutions for Better Health Care, 69910W (2 May 2008); doi: 10.1117/12.787043
Show Author Affiliations
Jonas Ries, Technische Univ. Dresden (Germany)
Christian Klose, MPI for Molecular Cell Biology and Genetics (Germany)
Christiane Walch-Solimena, Max Planck Society (Germany)
Petra Schwille, Technische Univ. Dresden (Germany)

Published in SPIE Proceedings Vol. 6991:
Biophotonics: Photonic Solutions for Better Health Care
Jürgen Popp; Wolfgang Drexler; Valery V. Tuchin; Dennis L. Matthews, Editor(s)

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