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Proceedings Paper

Rapid screening and species identification of E. coli, Listeria, and Salmonella by SERS technique
Author(s): Yongliang Liu; Kuanglin Chao; Moon S. Kim; Xiangwu Nou
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Paper Abstract

Techniques for routine and rapid screening of the presence of foodborne bacteria are needed, and this study reports the feasibility of citrate-reduced silver colloidal SERS for identifying E. coli, Listeria, and Salmonella. Relative standard deviation (RSD) of SERS spectra from silver colloidal suspensions and ratios of P-O SERS peaks from small molecule (K3PO4) were used to assess the reproducibility, stability, and binding effectiveness of citrate-reduced silver colloids over batch and storage process. The results suggested the reproducibility of silver colloids over batch process and also stability and consistent binding effectiveness over 60-day storage period. Notably, although silver colloidal nanoparticles were stable for at least 90 days, their binding effectiveness began to decrease slightly after 60-day storage, with a binding reduction of about 12% at 90th day. Colloidal silver SERS, as demonstrated here, could be an important alternative technique in the rapid and simultaneous screening of the presence of three most outbreak bacteria due to the exclusive biomarkers, label-free and easy sampling attribute.

Paper Details

Date Published: 18 April 2008
PDF: 9 pages
Proc. SPIE 6983, Defense and Security 2008: Special Sessions on Food Safety, Visual Analytics, Resource Restricted Embedded and Sensor Networks, and 3D Imaging and Display, 698309 (18 April 2008); doi: 10.1117/12.786881
Show Author Affiliations
Yongliang Liu, Univ. of Maryland, College Park (United States)
Kuanglin Chao, USDA Agricultural Research Service (United States)
Moon S. Kim, USDA Agricultural Research Service (United States)
Xiangwu Nou, USDA Agricultural Research Service (United States)


Published in SPIE Proceedings Vol. 6983:
Defense and Security 2008: Special Sessions on Food Safety, Visual Analytics, Resource Restricted Embedded and Sensor Networks, and 3D Imaging and Display
Moon S. Kim; Kaunglin Chao; William J. Tolone; William Ribarsky; Sergey I. Balandin; Bahram Javidi; Shu-I Tu, Editor(s)

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