Share Email Print
cover

Proceedings Paper

Rapid Raman microscopic imaging for potential histological screening
Author(s): Joanne Hutchings; Catherine Kendall; Neil Shepherd; Hugh Barr; Brian Smith; Nicholas Stone
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

Raman spectroscopy has proved to be a highly sensitive tool for differentiating between normal, cancerous or pre-cancerous tissues. To date, histological application of Raman mapping has been limited due to lengthy mapping times. StreamlineTM Raman imaging is a novel mapping technique that has reduced total mapping times to a level that is becoming clinically practicable. Raman Streamline mapping was carried out on a 20μm frozen section of an oesophageal biopsy. A contiguous 7μm section was stained with haematoxylin and eosin (H&E) with histpathology analysed by a pathologist. The step size and acquisition times were varied and the resulting spectra, principal component score maps and loads were compared. The signal to noise for the raw spectra and a relative 'signal to noise' of the principal component loads were determined. The Streamline mapping technique was also compared to traditional point Raman mapping. The principal component loads were similar despite varying the acquisition time and number of spectra, with the fifth load used for comparison of the noise levels. Gross biochemical information was extracted showing good correlation with the H&E section even for short overall mapping times as low as 30-90 minutes for a biopsy ~2mm in diameter (0.5s acquisition time per 25.3μm Raman pixel). Streamline mapping was of the order of 3-7 times faster than traditional point mapping with the greatest improvement made for high resolution maps. Further optimization of the system is still possible which will reduce this mapping time further making implementation in a clinical environment a future possibility.

Paper Details

Date Published: 8 February 2008
PDF: 9 pages
Proc. SPIE 6853, Biomedical Optical Spectroscopy, 685305 (8 February 2008); doi: 10.1117/12.786440
Show Author Affiliations
Joanne Hutchings, Gloucestershire Hospitals NHS Foundation Trust (United Kingdom)
Cranfield Univ. (United Kingdom)
Catherine Kendall, Gloucestershire Hospitals NHS Foundation Trust (United Kingdom)
Cranfield Univ. (United Kingdom)
Neil Shepherd, Gloucestershire Hospitals NHS Foundation Trust (United Kingdom)
Hugh Barr, Gloucestershire Hospitals NHS Foundation Trust (United Kingdom)
Cranfield Univ. (United Kingdom)
Brian Smith, Renishaw Plc. (United Kingdom)
Nicholas Stone, Gloucestershire Hospitals NHS Foundation Trust (United Kingdom)
Cranfield Univ. (United Kingdom)


Published in SPIE Proceedings Vol. 6853:
Biomedical Optical Spectroscopy
Anita Mahadevan-Jansen; Wolfgang Petrich; Robert R. Alfano; Alvin Katz, Editor(s)

© SPIE. Terms of Use
Back to Top