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Proceedings Paper

Axial resolution for two-photon wide-field illumination microscopy and microfabrication
Author(s): Daekeun Kim; Peter So
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Paper Abstract

For the last two decades, multiphoton excitation microscopy/microfabrication based on laser scanning/writing techniques has been popular in the life science as well as photonics. Due to the slow scanning/writing nature, these applications are very limited to the production of prototypes, although its submicron optical resolution and intrinsic 3D optical sectioning capability are very attractive for creating 3D structures. In this proceeding, we introduced multiphoton excitation microscopy and microfabrication based on wide-field illumination. We derived mathematical model for wide-field illumination in the microscopy and microfabrication, and identified the design parameters that affect axial resolution for the proposed system. The future work of developing optical model combined with photopolymerization is also discussed.

Paper Details

Date Published: 15 February 2008
PDF: 7 pages
Proc. SPIE 6860, Multiphoton Microscopy in the Biomedical Sciences VIII, 686028 (15 February 2008); doi: 10.1117/12.784748
Show Author Affiliations
Daekeun Kim, Massachusetts Institute of Technology (United States)
Peter So, Massachusetts Institute of Technology (United States)

Published in SPIE Proceedings Vol. 6860:
Multiphoton Microscopy in the Biomedical Sciences VIII
Ammasi Periasamy; Peter T. C. So, Editor(s)

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