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Proceedings Paper

Optimizing protocols for imaging neural cells and tissues using functionalized quantum dots
Author(s): Smita Pathak; Gabriel A. Silva
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Paper Abstract

Chemically functionalized semiconductor quantum dot protocols were optimized for the specific labeling and imaging of neural cells, both neurons and macroglial cells. Beta-tubulin III was used to image primary cortical neurons and PC12 cells while glial fibrillary acidic protein (GFAP) was used to image primary spinal cord and cortical astrocytes and the rMC-1 retinal glial Muller cell line. Both proteins are the main components of intermediate filaments and are specific to the two classes of neural cells. We also specifically labeled and imaged at high resolutions using anti-GFAP conjugated quantum dots glial scars in situ in intact neural sensory retina in a rodent model of macular degeneration.

Paper Details

Date Published: 26 February 2008
PDF: 11 pages
Proc. SPIE 6866, Colloidal Quantum Dots for Biomedical Applications III, 68660V (26 February 2008); doi: 10.1117/12.784337
Show Author Affiliations
Smita Pathak, Univ. of California, San Diego (United States)
Gabriel A. Silva, Univ. of California, San Diego (United States)

Published in SPIE Proceedings Vol. 6866:
Colloidal Quantum Dots for Biomedical Applications III
Marek Osinski; Thomas M. Jovin; Kenji Yamamoto, Editor(s)

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