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Proceedings Paper

New approach on evaluation of liposome release property with electric impedance measuring method
Author(s): Guoming Chen; Zhongwei Jiang; Makato Yoshimoto; Yafang Luo; Yunlong Wei
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Paper Abstract

The purpose of this study is to develop a novel method for evaluation of the liposomes' release property by detecting the electric impedance change in the liposome solution. Liposomes encapsulating calcein (calcein-liposomes) and de-ionized water (white-liposomes) were prepared and ultrasound was employed as the stimulus in order to release the calcein from liposomes. The impedance change of calcein-liposomes in de-ionized water was measured before and after ultrasound treatment, as well as that of white-liposomes in de-ionized water and in calcein solutions for comparison. Besides, the release of calcein-liposome suspensions was evaluated simultaneously by spectrofluorometer. Results showed that the impedance of white-liposomes would change greatly when it was exposed to ultrasound and it was related to the total ion concentration of the sample. The release rate of calcein calculated with electric impedances showed a good agreement to that calculated with fluorescence intensities. It indicates that the electric impedance measuring method has high potential to estimate the release rate as the fluorescence analysis do, and might be used for non-fluorescent encapsulated liposomes which could not be evaluated by fluorescence method.

Paper Details

Date Published: 9 January 2008
PDF: 6 pages
Proc. SPIE 6794, ICMIT 2007: Mechatronics, MEMS, and Smart Materials, 67943J (9 January 2008); doi: 10.1117/12.783960
Show Author Affiliations
Guoming Chen, Yamaguchi Univ. (Japan)
Chongqing Institute of Technology (China)
Zhongwei Jiang, Yamaguchi Univ. (Japan)
Makato Yoshimoto, Yamaguchi Univ. (Japan)
Yafang Luo, Yamaguchi Univ. (Japan)
Chongqing Institute of Technology (China)
Yunlong Wei, Chongqing Institute of Technology (China)


Published in SPIE Proceedings Vol. 6794:
ICMIT 2007: Mechatronics, MEMS, and Smart Materials

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