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Proceedings Paper

Contrast mechanisms and signal epidetection in THG microscopy of scattering tissues
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Paper Abstract

We use a vector field model to analyze the third-harmonic generation (THG) emission patterns for isolated objects illuminated by a Gaussian beam. Simulations and experiments indicate that THG from biological (dielectric) structures is essentially forward-directed, as opposed to e.g. THG from gold particles. We then address the issue of epidetecting forward-emitted light backscattered in a turbid medium. We use Monte Carlo simulations and measurements to analyze the effect of tissue properties (absorption, scattering), and of the geometry of the collecting optics. This analysis provides guidelines for optimizing epidetection in coherent nonlinear microscopy. An erratum is attached.

Paper Details

Date Published: 9 May 2008
PDF: 10 pages
Proc. SPIE 6991, Biophotonics: Photonic Solutions for Better Health Care, 699114 (9 May 2008); doi: 10.1117/12.783376
Show Author Affiliations
Nicolas Olivier, Lab. for Optics and Biosciences, Ecole Polytechnique, CNRS INSERM (France)
Delphine Débarre, Lab. for Optics and Biosciences, Ecole Polytechnique, CNRS INSERM (France)
Emmanuel Beaurepaire, Lab. for Optics and Biosciences, Ecole Polytechnique, CNRS INSERM (France)


Published in SPIE Proceedings Vol. 6991:
Biophotonics: Photonic Solutions for Better Health Care
Jürgen Popp; Wolfgang Drexler; Valery V. Tuchin; Dennis L. Matthews, Editor(s)

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