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Proceedings Paper

High resolution detection system of capillary electrophoresis
Author(s): Jie Wang; Li Qiang Wang; Yan Shi; Hua Zheng; Zu Kang Lu
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Paper Abstract

The capillary electrophoresis (CE) with laser induced fluorescence detection (LIFD) system was founded according to confocal theory. The 3-D adjustment of the exciting and collecting optical paths was realized. The photomultiplier tube (PMT) is used and the signals are processed by a software designed by ourselves. Under computer control, high voltage is applied to appropriate reservoirs and to inject and separate DNA samples respectively. Two fluorescent dyes Thiazole Orange (TO) and SYBR Green I were contrasted. With both of the dyes, high signals-to-noise images were obtained with the CE-LIFD system. The single-bases can be distinguished from the electrophoretogram and high resolution of DNA sample separation was obtained.

Paper Details

Date Published: 17 January 2008
PDF: 5 pages
Proc. SPIE 6723, 3rd International Symposium on Advanced Optical Manufacturing and Testing Technologies: Optical Test and Measurement Technology and Equipment, 67230K (17 January 2008); doi: 10.1117/12.782913
Show Author Affiliations
Jie Wang, Zhejiang Univ. (China)
Li Qiang Wang, Zhejiang Univ. (China)
Yan Shi, Zhejiang Univ. (China)
Hua Zheng, Zhejiang Univ. (China)
Fujian Normal Univ. (China)
Zu Kang Lu, Zhejiang Univ. (China)


Published in SPIE Proceedings Vol. 6723:
3rd International Symposium on Advanced Optical Manufacturing and Testing Technologies: Optical Test and Measurement Technology and Equipment
Junhua Pan; James C. Wyant; Hexin Wang, Editor(s)

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