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Proceedings Paper

Irradiation by pulsed Nd:YAG laser induces the production of extracellular matrix molecules by cells of the connective tissues: a tool for tissue repair
Author(s): Monica Monici; Venere Basile; Francesca Cialdai; Giovanni Romano; Franco Fusi; Antonio Conti
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Paper Abstract

Many studies demonstrated that mechanical stress is a key factor for tissue homeostasis, while unloading induce loss of mass and impairment of function. Because of their physiological function, muscle, connective tissue, bone and cartilage dynamically interact with mechanical and gravitational stress, modifying their properties through the continuous modification of their composition. Indeed, it is known that mechanical stress increases the production of extracellular matrix (ECM) components by cells, but the mechanotransduction mechanisms and the optimal loading conditions required for an optimal tissue homeostasis are still unknown. Considering the importance of cell activation and ECM production in tissue regeneration, a proper use of mechanical stimulation could be a powerful tool in tissue repair and tissue engineering. Studies exploring advanced modalities for supplying mechanical stimuli are needed to increase our knowledge on mechanobiology and to develop effective clinical applications. Here we describe the effect of photomechanical stress, supplied by a pulsed Nd:YAG laser on ECM production by cells of connective tissues. Cell morphology, production of ECM molecules (collagens, fibronectin, mucopolysaccharides), cell adhesion and cell energy metabolism have been studied by using immunofluorescence and autofluorescence microscopy. The results show that photomechanical stress induces cytoskeleton remodelling, redistribution of membrane integrins, increase in production of ECM molecules. These results could be of consequence for developing clinical protocols for the treatment of connective tissue dideases by pulsed Nd:YAG laser.

Paper Details

Date Published: 2 May 2008
PDF: 10 pages
Proc. SPIE 6991, Biophotonics: Photonic Solutions for Better Health Care, 69912K (2 May 2008); doi: 10.1117/12.782865
Show Author Affiliations
Monica Monici, ASAcampus, Univ. of Florence (Italy)
Venere Basile, Univ. of Florence (Italy)
Francesca Cialdai, Univ. of Florence (Italy)
Giovanni Romano, Univ. of Florence (Italy)
Franco Fusi, Univ. of Florence (Italy)
Antonio Conti, Univ. of Florence (Italy)

Published in SPIE Proceedings Vol. 6991:
Biophotonics: Photonic Solutions for Better Health Care
Jürgen Popp; Wolfgang Drexler; Valery V. Tuchin; Dennis L. Matthews, Editor(s)

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