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Proceedings Paper

Dynamically monitoring the gene expression of dual fluorophore in the cell cycle with quantitative spectrum analysis
Author(s): Ja-Yun Lee; Tzong-Yuan Wu; I-Jen Hsu
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Paper Abstract

The cloning and transcription techniques on gene cloned fluorescent proteins have been widely used in many applications. They have been used as reporters of some conditions in a series of reactions. However, it is usually difficult to monitor the specific target with the exactly number of proteins during the process in turbid media, especially at micrometer scales. We successfully revealed an alternative way to monitor the cell cycle behavior and quantitatively analyzed the target cells with green and red fluorescent proteins (GFP and RFP) during different phases of the cell cycle by quantitatively analyzing its behavior and also monitoring its spatial distribution.

Paper Details

Date Published: 2 May 2008
PDF: 9 pages
Proc. SPIE 6991, Biophotonics: Photonic Solutions for Better Health Care, 699113 (2 May 2008); doi: 10.1117/12.781602
Show Author Affiliations
Ja-Yun Lee, Chung Yuan Christian Univ. (Taiwan)
Tzong-Yuan Wu, Chung Yuan Christian Univ. (Taiwan)
I-Jen Hsu, Chung Yuan Christian Univ. (Taiwan)


Published in SPIE Proceedings Vol. 6991:
Biophotonics: Photonic Solutions for Better Health Care
Jürgen Popp; Wolfgang Drexler; Valery V. Tuchin; Dennis L. Matthews, Editor(s)

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