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Proceedings Paper

Confocal light scattering and absorption spectroscopic microscopy
Author(s): Le Qiu; Edward Vitkin; Saira Salahuddin; Munir M. Zaman; Charlotte Andersson; Steven D. Freedman; Eugene B. Hanlon; Irving Itzkan; Lev T. Perelman
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Paper Abstract

We have developed a novel optical method for observing submicron intracellular structures in living cells which is called confocal light absorption and scattering spectroscopic (CLASS) microscopy. It combines confocal microscopy, a well-established high-resolution microscopic technique, with light scattering spectroscopy (LSS). CLASS microscopy requires no exogenous labels and is capable of imaging and continuously monitoring individual viable cells, enabling the observation of cell and organelle functioning at scales on the order of 100 nm. In addition, it provides not only size information but also information about the biochemical and physical properties of the cell.

Paper Details

Date Published: 2 May 2008
PDF: 7 pages
Proc. SPIE 6991, Biophotonics: Photonic Solutions for Better Health Care, 69910X (2 May 2008); doi: 10.1117/12.781562
Show Author Affiliations
Le Qiu, Harvard Univ. (United States)
Edward Vitkin, Harvard Univ. (United States)
Saira Salahuddin, Harvard Univ. (United States)
Munir M. Zaman, Harvard Univ. (United States)
Charlotte Andersson, Harvard Univ. (United States)
Steven D. Freedman, Harvard Univ. (United States)
Eugene B. Hanlon, Harvard Univ. (United States)
Irving Itzkan, Harvard Univ. (United States)
Lev T. Perelman, Harvard Univ. (United States)

Published in SPIE Proceedings Vol. 6991:
Biophotonics: Photonic Solutions for Better Health Care
Jürgen Popp; Wolfgang Drexler; Valery V. Tuchin; Dennis L. Matthews, Editor(s)

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