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Proceedings Paper

Design of specific biochips for contrast enhancement of UV biological absorption
Author(s): Kristelle Robin; Jean-Luc Reverchon; Laurent Mugherli; Henri Benisty
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Paper Abstract

This paper describes the design of biochips enhancing absorption in UV (and more particularly at 260-280 nm). These contrast-enhancing multilayers structures are developed in order to image biological compounds immobilized at a surface. The measurement set-up is a reflection set-up, including a previously developed AlGaN detector, spectrally selective at the wavelength of biological compounds absorption (260 nm for DNA and 280 nm for proteins). A contrast study is carried out using an inorganic absorber (titanium dioxide), enabling an optimized electromagnetic interaction with the absorber. The biochip design is based on an aluminum mirror covered with a transparent dielectric. To account for roughness and oxidation, the material is modeled by a thin layer of effective medium defined in the Bruggeman approximation. We discuss the absorption expected from various biological compounds, and the capability of our set-up for detection of monolayers of DNA or protein molecules.

Paper Details

Date Published: 9 May 2008
PDF: 10 pages
Proc. SPIE 6991, Biophotonics: Photonic Solutions for Better Health Care, 69912A (9 May 2008); doi: 10.1117/12.781452
Show Author Affiliations
Kristelle Robin, Thales Research and Technology (France)
Jean-Luc Reverchon, Thales Research and Technology (France)
Laurent Mugherli, Lab. de Biochimie, Ecole Polytechnique, CNRS (France)
Henri Benisty, Lab. Charles Fabry de l'Institut d'Optique (France)

Published in SPIE Proceedings Vol. 6991:
Biophotonics: Photonic Solutions for Better Health Care
Jürgen Popp; Wolfgang Drexler; Valery V. Tuchin; Dennis L. Matthews, Editor(s)

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