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Proceedings Paper

Identification of active fluorescence stained bacteria by Raman spectroscopy
Author(s): Mario Krause; Beatrice Beyer; Christian Pietsch; Benno Radt; Michaela Harz; Petra Rösch; Jürgen Popp
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Paper Abstract

Microorganisms can be found everywhere e.g. in food both as useful ingredients or harmful contaminations causing food spoilage. Therefore, a fast and easy to handle analysis method is needed to detect bacteria in different kinds of samples like meat, juice or air to decide if the sample is contaminated by harmful microorganisms. Conventional identification methods in microbiology require always cultivation and therefore are time consuming. In this contribution we present an analysis approach to identify fluorescence stained bacteria on strain level by means of Raman spectroscopy. The stained bacteria are highlighted and can be localized easier against a complex sample environment e.g. in food. The use of Raman spectroscopy in combination with chemometrical methods allows the identification of single bacteria within minutes.

Paper Details

Date Published: 2 May 2008
PDF: 8 pages
Proc. SPIE 6991, Biophotonics: Photonic Solutions for Better Health Care, 69910E (2 May 2008); doi: 10.1117/12.781321
Show Author Affiliations
Mario Krause, Univ. of Jena (Germany)
Beatrice Beyer, Univ. of Jena (Germany)
Christian Pietsch, Univ. of Jena (Germany)
Benno Radt, Carl Zeiss AG (Germany)
Michaela Harz, Univ. of Jena (Germany)
Petra Rösch, Univ. of Jena (Germany)
Jürgen Popp, Univ. of Jena (Germany)
Institute for Photonic Technology (Germany)

Published in SPIE Proceedings Vol. 6991:
Biophotonics: Photonic Solutions for Better Health Care
Jürgen Popp; Wolfgang Drexler; Valery V. Tuchin; Dennis L. Matthews, Editor(s)

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