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Proceedings Paper

Specific peptide for functionalization of GaN
Author(s): E. Estephan; C. Larroque; T. Cloitre; F. J. G. Cuisinier; C. Gergely
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Paper Abstract

Nanobiotechnology aims to exploit biomolecular recognition and self-assembly capabilities for integrating advanced materials into medicine and biology. However frequent problems are encountered at the interface of substrate-biological molecule, as the direct physical adsorption of biological molecules is dependent of unpredictable non-specific interactions with the surface, often causing their denaturation. Therefore, a proper functionalization of the substrate should avoid a loss of biological activity. In this work we address the functionalization of the semiconductor GaN (0001) for biosensing applications. The basic interest of using III-V class semiconductors is their good light emitting properties and a fair chemical stability that allows various applications of these materials. The technology chosen to elaborate GaN-specific peptides is the combinatorial phage-display method, a biological screening procedure based on affinity selection. An M13 bacteriophage library has been used to screen 1010 different peptides against the GaN (0001) surface to finally isolate one specific peptide. The preferential attachment of the biotinylated selected peptide onto the GaN (0001), in close proximity to a surface of different chemical and structural composition has been demonstrated by fluorescence microscopy. Further physicochemical studies have been initiated to evaluate the semiconductor-peptide interface and understand the details in the specific recognition of peptides for semiconductor substrates. Fourier Transform Infrared spectroscopy in Attenuated Total Reflection mode (FTIR-ATR) has been employed to prove the presence of peptides on the surface. Our Atomic Force Microscopy (AFM) studies on the morphology of the GaN surface after functionalization revealed a total surface coverage by a very thin, homogeneous peptide layer. Due to its good biocompatibility, functionalized GaN devices might evolve in a new class of implantable biosensors for medical applications.

Paper Details

Date Published: 8 May 2008
PDF: 8 pages
Proc. SPIE 6991, Biophotonics: Photonic Solutions for Better Health Care, 699121 (8 May 2008); doi: 10.1117/12.781205
Show Author Affiliations
E. Estephan, Groupe d’Etude des Semi-conducteurs, CNRS, Univ. Montpellier II (France)
C. Larroque, Univ. Montpellier I (France)
T. Cloitre, Groupe d’Etude des Semi-conducteurs, CNRS, Univ. Montpellier II (France)
F. J. G. Cuisinier, Univ. Montpellier I (France)
C. Gergely, Groupe d’Etude des Semi-conducteurs, CNRS, Univ. Montpellier II (France)

Published in SPIE Proceedings Vol. 6991:
Biophotonics: Photonic Solutions for Better Health Care
Jürgen Popp; Wolfgang Drexler; Valery V. Tuchin; Dennis L. Matthews, Editor(s)

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