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Proceedings Paper

Optical detection of PNA/DNA hybridization in resonant porous silicon-based devices
Author(s): Lucia Rotiroti; Paolo Arcari; Annalisa Lamberti; Carmen Sanges; Edoardo De Tommasi; Ilaria Rea; Ivo Rendina; Luca De Stefano
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Paper Abstract

The development of label-free optical biosensors could have a great impact on life sciences as well as on screening techniques for medical and environmental applications. Peptide nucleic acid (PNA) is a nucleic acid analog in which the sugar phosphate backbone of natural nucleic acid has been replaced by a synthetic peptide backbone, resulting in an achiral and uncharged mimic. Due to the uncharged nature of PNA, PNA-DNA duplexes show a better thermal stability respect the DNA-DNA equivalents. In this work, we used an optical biosensor, based on the porous silicon (PSi) nanotechnology, to detect PNA-DNA interactions. PSi optical sensors are based on changes of reflectivity spectrum when they are exposed to the target analytes. The porous silicon surface was chemically modified to covalently link the PNA which acts as a very specific probe for its ligand (cDNA).

Paper Details

Date Published: 8 May 2008
PDF: 7 pages
Proc. SPIE 6991, Biophotonics: Photonic Solutions for Better Health Care, 699120 (8 May 2008); doi: 10.1117/12.781147
Show Author Affiliations
Lucia Rotiroti, Institute for Microelectronics and Microsystems, CNR (Italy)
Federico II Univ. of Naples (Italy)
Paolo Arcari, Federico II Univ. of Naples (Italy)
Annalisa Lamberti, Federico II Univ. of Naples (Italy)
Carmen Sanges, Federico II Univ. of Naples (Italy)
Edoardo De Tommasi, Institute for Microelectronics and Microsystems, CNR (Italy)
Ilaria Rea, Institute for Microelectronics and Microsystems, CNR (Italy)
Federico II Univ. of Naples (Italy)
Ivo Rendina, Institute for Microelectronics and Microsystems, CNR (Italy)
Luca De Stefano, Institute for Microelectronics and Microsystems, CNR (Italy)


Published in SPIE Proceedings Vol. 6991:
Biophotonics: Photonic Solutions for Better Health Care
Jürgen Popp; Wolfgang Drexler; Valery V. Tuchin; Dennis L. Matthews, Editor(s)

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