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Proceedings Paper

Determination of the integral refractive index of cells in suspension by digital holographic phase contrast microscopy
Author(s): Sebastian Kosmeier; Björn Kemper; Patrik Langehanenberg; Ilona Bredebusch; Jürgen Schnekenburger; Andreas Bauwens; Gert von Bally
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Paper Abstract

A digital holographic microscopy method for the determination of the integral refractive index of living cells in suspension is presented. Therefore, digital holographic phase contrast images of trypsinized cells in suspension are recorded. Afterwards, the cell radius and the integral cellular refractive index are determined by fitting of a two dimensional model. The applied algorithm requires only information about the refractive index of the suspension medium and the scale of the microscopic imaging system. The fitting algorithm is characterized on simulated phase data and digital holographic phase contrast images of beads. Results obtained from living human pancreas tumor cells demonstrate the applicability of the method for cell characterization.

Paper Details

Date Published: 2 May 2008
PDF: 8 pages
Proc. SPIE 6991, Biophotonics: Photonic Solutions for Better Health Care, 699110 (2 May 2008); doi: 10.1117/12.781125
Show Author Affiliations
Sebastian Kosmeier, Univ. Münster (Germany)
Björn Kemper, Univ. Münster (Germany)
Patrik Langehanenberg, Univ. Münster (Germany)
Ilona Bredebusch, Univ. Münster (Germany)
Jürgen Schnekenburger, Univ. Münster (Germany)
Andreas Bauwens, Univ. Münster (Germany)
Gert von Bally, Univ. Münster (Germany)


Published in SPIE Proceedings Vol. 6991:
Biophotonics: Photonic Solutions for Better Health Care
Jürgen Popp; Wolfgang Drexler; Valery V. Tuchin; Dennis L. Matthews, Editor(s)

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