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Proceedings Paper

Development of cell-based quantitative evaluation method for cell cycle-arrest type cancer drugs for apoptosis by high precision surface plasmon resonance sensor
Author(s): Toshihiro Ona; Hiroshi Nishijima; Atsushi Kosaihira; Junko Shibata
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Paper Abstract

In vitro rapid and quantitative cell-based assay is demanded to verify the efficacy prediction of cancer drugs since a cancer patient may have unconventional aspects of tumor development. Here, we show the rapid and non-label quantitative verifying method and instrumentation of apoptosis for cell cycle-arrest type cancer drugs (Roscovitine and D-allose) by reaction analysis of living liver cancer cells cultured on a sensor chip with a newly developed high precision (50 ndeg s-1 average fluctuation) surface plasmon resonance (SPR) sensor. The time-course cell reaction as the SPR angle change rate for 10 min from 30 min cell culture with a drug was significantly related to cell viability. By the simultaneous detection of differential SPR angle change and fluorescence by specific probes using the new instrument, the SPR angle was related to the nano-order potential decrease in inner mitochondrial membrane potential. The results obtained are universally valid for the cell cycle-arrest type cancer drugs, which mediate apoptosis through different cell-signaling pathways, by a liver cancer cell line of Hep G2 (P<0.001). This system towards the application to evaluate personal therapeutic potentials of drugs using cancer cells from patients in clinical use.

Paper Details

Date Published: 2 May 2008
PDF: 8 pages
Proc. SPIE 6991, Biophotonics: Photonic Solutions for Better Health Care, 69910R (2 May 2008); doi: 10.1117/12.780819
Show Author Affiliations
Toshihiro Ona, Kyushu Univ. (Japan)
Hiroshi Nishijima, Kyushu Univ. (Japan)
Atsushi Kosaihira, Kyushu Univ. (Japan)
Junko Shibata, System Instruments Co., Ltd. (Japan)

Published in SPIE Proceedings Vol. 6991:
Biophotonics: Photonic Solutions for Better Health Care
Jürgen Popp; Wolfgang Drexler; Valery V. Tuchin; Dennis L. Matthews, Editor(s)

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