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Proceedings Paper

Fluorescence resonance energy transfer between CdTe semiconductor nanocrystals donors and gold nanoparticles labeled DNA acceptors
Author(s): Zhao Dai; Jimei Zhang; Shichao Xu; Ning Guo; Yuehua Bu; Quanxi Dong
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Paper Abstract

CdTe semiconductor nanocrystals with 2.5 nm in diameters, as energy donors, were prepared in water. Narrow-disperse Au nanoparticles with 16 nm in diameters, as energy acceptors, were prepared from gold chloride by reduction. It was confirmed that the emission spectrum of CdTe ODs and the absorption spectrum of AuNPs had an appreciable overlap. Therefore, a DNA fluorescence probe system based on fluorescence resonance energy transfer (FRET) from CdTe quantum dots donors to gold nanoparticles acceptors was designed. CdTe QDs were linked to 5'-NH2-DNA through 1- ethyl-3-(dimethylaminopr opyl) carbodi-imide hydrochloride (EDC) as a linker, and the 3'-SH-DNA was self-assembled onto the surface of Au nanoparticles. The hybridization of complementary DNA bound to the QDs and Au nanoparticles (CdTe-DNA-Au) determined the FRET distance of CdTe QDs and Au nanoparticles. Compared with the fluorescence of CdTe-DNA, the fluorescence of CdTe-DNA-Au conjugates decreased extremely, which indicated that the FRET occurred between CdTe ODs and Au nanoparticles. The fluorescence change of this conjugates was depended on the ratio of Au-DNA and CdTe-DNA, when the AuNPs-DNA to QD-DNA ratio is 5:1, the FRET efficiency reached a maximum. If a complementary single stranded DNA was add to the CdTe-DNA-Au conjugates, the fluorescence of the probe system would have a certain degree recovery, this showed that the distance between CdTe QDs and Au nanoparticles was increased.

Paper Details

Date Published: 1 November 2007
PDF: 6 pages
Proc. SPIE 6423, International Conference on Smart Materials and Nanotechnology in Engineering, 642357 (1 November 2007); doi: 10.1117/12.780662
Show Author Affiliations
Zhao Dai, Tianjin Polytechnic Univ. (China)
Jimei Zhang, Tianjin Polytechnic Univ. (China)
Shichao Xu, Tianjin Polytechnic Univ. (China)
Ning Guo, Tianjin Polytechnic Univ. (China)
Yuehua Bu, Tianjin Polytechnic Univ. (China)
Quanxi Dong, Tianjin Polytechnic Univ. (China)

Published in SPIE Proceedings Vol. 6423:
International Conference on Smart Materials and Nanotechnology in Engineering
Shanyi Du; Jinsong Leng; Anand K. Asundi, Editor(s)

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