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Proceedings Paper

Fluorescence and absorbance measurements for chromatographic analysis using a miniaturized micro-optical detection unit
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Paper Abstract

We present a micro-optical detection unit for both laser induced fluorescence and absorbance analysis in fused silica capillaries, which can be used for chromatographic applications. The detection system is designed by means of non-sequential ray tracing simulations and prototyped by means of Deep Proton Writing. Such a prototyped master component is afterwards replicated by means of elastomeric moulding and vacuum casting. In a proof-of-concept demonstration the prototyped master micro-optical unit is used for the detection of various concentrations of coumarin dyes. The detection limit (SNR=3.3) achieved measures 0.6nM for fluorescence analysis and 12μM for absorbance measurements in capillaries with an inner diameter of 150μm. We discuss the optimization of different measurement parameters of the detectors in the setup in order to achieve accurate, fast and sensitive measurements.

Paper Details

Date Published: 2 May 2008
PDF: 12 pages
Proc. SPIE 6991, Biophotonics: Photonic Solutions for Better Health Care, 69910V (2 May 2008); doi: 10.1117/12.780490
Show Author Affiliations
Sara Van Overmeire, Vrije Univ. Brussel (Belgium)
Heidi Ottevaere, Vrije Univ. Brussel (Belgium)
Gert Desmet, Vrije Univ. Brussel (Belgium)
Hugo Thienpont, Vrije Univ. Brussel (Belgium)


Published in SPIE Proceedings Vol. 6991:
Biophotonics: Photonic Solutions for Better Health Care
Jürgen Popp; Wolfgang Drexler; Valery V. Tuchin; Dennis L. Matthews, Editor(s)

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