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Proceedings Paper

Fluorescence diffuse optical tomography measurements for tissue engineering
Author(s): Johanne Desrochers; Patrick Vermette; Réjean Fontaine; Yves Bérubé-Lauzière
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Paper Abstract

Currents eorts in tissue engineering (TE) are directed towards growing 3D volumes of tissues. In response to TE needs, we are developing a non-invasive technique based on fluorescence diuse optical tomography (FDOT) to image in 3D, via fluorescence labelling, the formation of micro-blood vessels in tissue cultures grown on biodegradable scaolds in bioreactor conditions. In the present work, we use a non-contact FDOT setup developed for small animal imaging for our measurements. We present experimental results showing the feasability to localize a fluorophore-filled 500μm capillary immersed in a scattering medium contained in a cylindrically-shaped glass tube. These conditions are representative of experiments to be carried on real tissue cultures. Time-resolved scattering-fluorescence measurements are made via Time-Correlated Single Photon Counting (TCSPC) and we use numerical constant fraction discrimination (NCFD) to obtain primary localization information from our time-resolved data.

Paper Details

Date Published: 26 October 2007
PDF: 8 pages
Proc. SPIE 6796, Photonics North 2007, 679607 (26 October 2007); doi: 10.1117/12.778939
Show Author Affiliations
Johanne Desrochers, Univ. de Sherbrooke (Canada)
Patrick Vermette, Univ. de Sherbrooke (Canada)
Réjean Fontaine, Univ. de Sherbrooke (Canada)
Yves Bérubé-Lauzière, Univ. de Sherbrooke (Canada)

Published in SPIE Proceedings Vol. 6796:
Photonics North 2007
John Armitage, Editor(s)

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