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Proceedings Paper

The optical biomedical sensors for DNA detection and imaging based on two-photon excited luminescent styryl dyes: phototoxic influence on the DNA
Author(s): Valeriy M. Yashchuk; Vladislav Yu. Kudrya; Mykhaylo Yu. Losytskyy; Valentyna P. Tokar; Sergiy M. Yarmoluk; Igor M. Dmytruk; Vadym M. Prokopets; Vladyslava B. Kovalska; Anatoliy O. Balanda; Dmytro V. Kryvorotenko; Tymish Yu. Ogul'chansky
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Paper Abstract

The optical absorption, fluorescence and phosphorescence of the novel styryl dyes developed for the fluorescent detection of DNA were investigated. The energy structures of dye molecules as well as spectral manifestations of the dyes aggregate formation and interaction with DNA were studied. The dramatic increase (up to 1000 times) of the fluorescence intensity of dyes in the presence of DNA was observed. The photostability and phototoxic influence on the DNA of several styryl dyes were studied by analyzing absorption, fluorescence and phosphorescence spectra of these dyes and dye-DNA systems. Changes of the optical density value of dye-DNA solutions caused by the visible light irradiation were fixed in the wavelength regions of the DNA absorption and of the dye absorption. Fluorescence emission of dye-DNA complexes upon two-photon excitation (TPE) at wavelength 1064 nm with the 20 ns pulsed YAG: Nd3+ laser and at 840 nm with the 90 fs pulsed Ti:sapphire laser was registered. The values of two-photon absorption cross-sections of dye-DNA complexes were evaluated.

Paper Details

Date Published: 8 November 2007
PDF: 14 pages
Proc. SPIE 6796, Photonics North 2007, 67960M (8 November 2007); doi: 10.1117/12.778096
Show Author Affiliations
Valeriy M. Yashchuk, Kyiv Taras Shevchenko National Univ. (Ukraine)
Vladislav Yu. Kudrya, Kyiv Taras Shevchenko National Univ. (Ukraine)
National Agricultural Univ. of Ukraine (Ukraine)
Mykhaylo Yu. Losytskyy, Kyiv Taras Shevchenko National Univ. (Ukraine)
Institute of Molecular Biology and Genetics of NASU (Ukraine)
Valentyna P. Tokar, Kyiv Taras Shevchenko National Univ. (Ukraine)
Institute of Molecular Biology and Genetics of NASU (Ukraine)
Sergiy M. Yarmoluk, Institute of Molecular Biology and Genetics of NASU (Ukraine)
Igor M. Dmytruk, Kyiv Taras Shevchenko National Univ. (Ukraine)
Vadym M. Prokopets, Kyiv Taras Shevchenko National Univ. (Ukraine)
Vladyslava B. Kovalska, Institute of Molecular Biology and Genetics of NASU (Ukraine)
Anatoliy O. Balanda, Institute of Molecular Biology and Genetics of NASU (Ukraine)
Dmytro V. Kryvorotenko, Institute of Molecular Biology and Genetics of NASU (Ukraine)
Tymish Yu. Ogul'chansky, SUNY/Buffalo (United States)


Published in SPIE Proceedings Vol. 6796:
Photonics North 2007

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