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Proceedings Paper

Methods and applications for marker-free quantitative digital holographic phase contrast imaging in life cell analysis
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Paper Abstract

Holographic interferometric metrology is an established technique for technical non-destructive testing. In connection with microscopy, digital holography provides contact-less and marker-free quantitative phase contrast imaging. The reconstruction of digitally captured holograms is performed numerically. Thus, multi-focus imaging of different object planes is achieved from a single captured hologram. In combination with (subsequent) numerical autofocus adjustment, this feature opens up applications for microscopic time-lapse investigations by avoidance of mechanical focus-maintenance. Results obtained from digital holographic investigations of toxin induced reactions of adherent cancer cells demonstrate applications prospects of digital holographic microscopy in quantitative life cell imaging. Furthermore, data obtained by cell spreading process analysis, the cell response to osmotic changes and the observation of shape variations of human erythrocytes supplies new information in marker-free life cell imaging.

Paper Details

Date Published: 26 October 2007
PDF: 8 pages
Proc. SPIE 6796, Photonics North 2007, 67960E (26 October 2007); doi: 10.1117/12.778093
Show Author Affiliations
Björn Kemper, Univ. of Muenster (Germany)
Patrik Langehanenberg, Univ. of Muenster (Germany)
Gert von Bally, Univ. of Muenster (Germany)

Published in SPIE Proceedings Vol. 6796:
Photonics North 2007
John Armitage, Editor(s)

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