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Proceedings Paper

Multiphoton microscopy imaging of collagen fiber layers and orientation in the tympanic membrane
Author(s): R. P. Jackson; C. Chlebicki; T. B. Krasieva; S. Puria
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Paper Abstract

Greater understanding of tympanic membrane (TM) biomechanics has the potential to guide future advances in medical technology related to its surgical repair (myringoplasty). The pars tensa of the TM is a composite structure with two collagen fiber layers that provide the main scaffolding for the TM. The external layer is arranged in an approximately radial configuration, and the other is arranged in an approximately circumferential configuration. A more detailed knowledge of collagen fiber orientation and volume fraction could greatly improve existing mechanical simulations of the TM. To address this, we employed multiphoton microscopy (MPM) imaging of the TM in two modalities: second harmonic generation (SHG) and two-photon fluorescence (TPF). The unique spectral signature of SHG allows selective imaging of collagen fibers. TPF also produces images of fibrillar-type collagen but lacks the specificity of SHG. Both the SHG and TPF images show patterns of collagen organization in the TM that match expected results with respect to both orientation and size. Through MPM, we intend to accurately determine the collagen fiber layer thickness, density, and orientation as a function of radial position and quadrant location.

Paper Details

Date Published: 8 February 2008
PDF: 8 pages
Proc. SPIE 6842, Photonic Therapeutics and Diagnostics IV, 68421D (8 February 2008); doi: 10.1117/12.774353
Show Author Affiliations
R. P. Jackson, Stanford Univ. (United States)
C. Chlebicki, Beckman Laser Institute (United States)
T. B. Krasieva, Beckman Laser Institute (United States)
S. Puria, Stanford Univ. (United States)

Published in SPIE Proceedings Vol. 6842:
Photonic Therapeutics and Diagnostics IV
Kenton W. Gregory; Guillermo J. Tearney; Reza S. Malek; Nikiforos Kollias; Bernard Choi; Haishan Zeng; Brian Jet-Fei Wong; Justus F. R. Ilgner; Henry Hirschberg; Steen J. Madsen, Editor(s)

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