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Proceedings Paper

Applying adaptive optics to three-dimensional wide-field microscopy
Author(s): Peter Kner; John Sedat; David Agard; Zvi Kam
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Paper Abstract

Depth aberrations are a major source of image degradation in three-dimensional microscopy, causing a significant loss of resolution and intensity deep into the sample. These aberrations occur because of an inevitable mismatch between the sample refractive index and the immersion medium index. We have built a wide-field fluorescence microscope that incorporates a large-throw deformable mirror to correct for depth aberrations in 3D imaging. We demonstrate a corrected point spread function imaging beads in water with an oil immersion lens and a twofold improvement in peak signal intensity. We apply this new microscope to imaging biological samples, and show sharper images and improved deconvolution.

Paper Details

Date Published: 8 February 2008
PDF: 12 pages
Proc. SPIE 6888, MEMS Adaptive Optics II, 688809 (8 February 2008); doi: 10.1117/12.773731
Show Author Affiliations
Peter Kner, Univ. of California, San Francisco (United States)
John Sedat, Univ. of California, San Francisco (United States)
David Agard, Univ. of California, San Francisco (United States)
Zvi Kam, Weizmann Institute of Science (Israel)

Published in SPIE Proceedings Vol. 6888:
MEMS Adaptive Optics II
Scot S. Olivier; Thomas G. Bifano; Joel A. Kubby, Editor(s)

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