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Proceedings Paper

Whole mouse cryo-imaging
Author(s): David Wilson; Debashish Roy; Grant Steyer; Madhusudhana Gargesha; Meredith Stone; Eliot McKinley
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Paper Abstract

The Case cryo-imaging system is a section and image system which allows one to acquire micron-scale, information rich, whole mouse color bright field and molecular fluorescence images of an entire mouse. Cryo-imaging is used in a variety of applications, including mouse and embryo anatomical phenotyping, drug delivery, imaging agents, metastastic cancer, stem cells, and very high resolution vascular imaging, among many. Cryo-imaging fills the gap between whole animal in vivo imaging and histology, allowing one to image a mouse along the continuum from the mouse -> organ -> tissue structure -> cell -> sub-cellular domains. In this overview, we describe the technology and a variety of exciting applications. Enhancements to the system now enable tiled acquisition of high resolution images to cover an entire mouse. High resolution fluorescence imaging, aided by a novel subtraction processing algorithm to remove sub-surface fluorescence, makes it possible to detect fluorescently-labeled single cells. Multi-modality experiments in Magnetic Resonance Imaging and Cryo-imaging of a whole mouse demonstrate superior resolution of cryo-images and efficiency of registration techniques. The 3D results demonstrate the novel true-color volume visualization tools we have developed and the inherent advantage of cryo-imaging in providing unlimited depth of field and spatial resolution. The recent results continue to demonstrate the value cryo-imaging provides in the field of small animal imaging research.

Paper Details

Date Published: 20 March 2008
PDF: 9 pages
Proc. SPIE 6916, Medical Imaging 2008: Physiology, Function, and Structure from Medical Images, 69161I (20 March 2008); doi: 10.1117/12.772840
Show Author Affiliations
David Wilson, Case Western Reserve Univ. (United States)
Univ. Hospitals of Cleveland (United States)
Debashish Roy, Case Western Reserve Univ. (United States)
Grant Steyer, Case Western Reserve Univ. (United States)
Madhusudhana Gargesha, Case Western Reserve Univ. (United States)
Meredith Stone, Case Western Reserve Univ. (United States)
Eliot McKinley, Case Western Reserve Univ. (United States)


Published in SPIE Proceedings Vol. 6916:
Medical Imaging 2008: Physiology, Function, and Structure from Medical Images
Xiaoping P. Hu; Anne V. Clough, Editor(s)

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