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Proceedings Paper

Multispectral image enhancement for H&E stained pathological tissue specimens
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Paper Abstract

The presence of a liver disease such as cirrhosis can be determined by examining the proliferation of collagen fiber from a tissue slide stained with special stain such as the Masson's trichrome(MT) stain. Collagen fiber and smooth muscle, which are both stained the same in an H&E stained slide, are stained blue and pink respectively in an MT-stained slide. In this paper we show that with multispectral imaging the difference between collagen fiber and smooth muscle can be visualized even from an H&E stained image. In the method M KL bases are derived using the spectral data of those H&E stained tissue components which can be easily differentiated from each other, i.e. nucleus, cytoplasm, red blood cells, etc. and based on the spectral residual error of fiber weighting factors are determined to enhance spectral features at certain wavelengths. Results of our experiment demonstrate the capability of multispectral imaging and its advantage compared to the conventional RGB imaging systems to delineate tissue structures with subtle colorimetric difference.

Paper Details

Date Published: 15 March 2008
PDF: 11 pages
Proc. SPIE 6918, Medical Imaging 2008: Visualization, Image-Guided Procedures, and Modeling, 691836 (15 March 2008); doi: 10.1117/12.771046
Show Author Affiliations
Pinky A. Bautista, Tokyo Institute of Technology (Japan)
Tokiya Abe, Tokyo Institute of Technology (Japan)
Masahiro Yamaguchi, Tokyo Institute of Technology (Japan)
Nagaaki Ohyama, Tokyo Institute of Technology (Japan)
Yukako Yagi, Harvard Univ. (United States)

Published in SPIE Proceedings Vol. 6918:
Medical Imaging 2008: Visualization, Image-Guided Procedures, and Modeling
Michael I. Miga; Kevin Robert Cleary, Editor(s)

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