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Proceedings Paper

Single molecule photophysics near metallic nanostructures
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Paper Abstract

Metal-enhanced fluorescence (MEF) is useful in single molecule detection (SMD) by increasing the photostability, brightness and increase in radiative decay rates of fluorophores. We have investigated MEF from an individual fluorophore tethered to a single silver nanoparticle and also a single fluorophore between a silver dimer. The fluorescence lifetime results revealed a near-field interaction mechanism of fluorophore with the metal particle. Finite-difference time-domain (FDTD) calculations were employed to study the distribution of electric field near the metal monomer and dimer. The coupling effect of metal particles on the fluorescence enhancement was studied. We have also investigated the photophysics of FRET near metal nanoparticles and our preliminary results suggest an enhanced FRET efficiency in the presence of a metal nanoparticle. In total, our results demonstrate improved detectability at the single molecule level for a variety of fluorophores and quantum dots in proximity to the silver nanoparticles due to the near-field metal-fluorophore interactions.

Paper Details

Date Published: 21 February 2008
PDF: 10 pages
Proc. SPIE 6862, Single Molecule Spectroscopy and Imaging, 68620S (21 February 2008); doi: 10.1117/12.770381
Show Author Affiliations
Jian Zhang, Univ. of Maryland School of Medicine (United States)
Yi Fu, Univ. of Maryland School of Medicine (United States)
Krishanu Ray, Univ. of Maryland School of Medicine (United States)
Mustafa H. Chowdhury, Univ. of Maryland School of Medicine (United States)
Henryk Szmacinski, Univ. of Maryland School of Medicine (United States)
Kazimierz Nowaczyk, Univ. of Maryland School of Medicine (United States)
Joseph R. Lakowicz, Univ. of Maryland School of Medicine (United States)


Published in SPIE Proceedings Vol. 6862:
Single Molecule Spectroscopy and Imaging
Jörg Enderlein; Zygmunt K. Gryczynski; Rainer Erdmann, Editor(s)

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