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Proceedings Paper

Use of adaptive optics to increase nonlinear imaging signal in mouse bone morrow
Author(s): Yaopeng Zhou; Thomas Bifano; Charles Lin
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Paper Abstract

In a recent effort, researchers from Wellman Center of Photomedicine use fluorescence signal provided by single- or two-photon excitation, second harmonic generation and coherent anti-Stokes Raman spectroscopy (CARS) to illustrate the cell level detail of mouse bone marrow [1]. However, the several non-linear imaging techniques suffered on a common base: signal degradation with deeper light penetration. The fluorescence signal weakening from the mouse skull is caused by the decreased excitation light intensity. With deeper imaging depth, the excitation light suffers tissue scattering, absorption and optical aberration. The last one of the causes spreads the light intensity away from its diffraction limited focal spot. In consequence, less fluorescence light is produced in the enlarged focal volume. In this paper, I will introduce Adaptive Optics (AO), a system for real time optical aberration compensation, to improve the non-linear fluorescence signal in the mouse bone marrow imaging. A parallel stochastic gradient decent algorithm based on Zernike polynomial is employed to control the deformable mirror in real time aberration compensation.

Paper Details

Date Published: 8 February 2008
PDF: 9 pages
Proc. SPIE 6888, MEMS Adaptive Optics II, 688808 (8 February 2008); doi: 10.1117/12.769506
Show Author Affiliations
Yaopeng Zhou, Boston Univ. (United States)
Thomas Bifano, Boston Univ. (United States)
Charles Lin, Wellman Ctr. for Photomedicine (United States)

Published in SPIE Proceedings Vol. 6888:
MEMS Adaptive Optics II
Scot S. Olivier; Thomas G. Bifano; Joel A. Kubby, Editor(s)

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