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Proceedings Paper

A laser-induced fluorescence optical fiber detection system for micro-fluidic chip
Author(s): Hong Wang; Yu Tang
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Paper Abstract

Based on optic fiber beam sensor, a miniaturized laser induced fluorescence optical fiber detection system using solid-state laser (473nm) as excitation source is developed. The advantage of the LIF-D system are simple structure, small size, low cost, high sensitivity and high anti-disturbing. And the photon count method is used to improve the sensitivity of the detection. Using Sodium fluorescein as the test sample, distinct fluorescence signal with good repeatability is showed in the experiment. The concentration detection limit is up to 3×10-13mol/L. the linear dynamic range is from 10-6 to 10-13 mol/L. It also shown that there was a nicer linear relation between the peak value of fluorescence signal to Sodium fluorescein concentration. Its relation coefficient is 0.99954. By changing detection cells this integrated detection system can be applied in micro flow analysis and for the normal biotechnology analysis of DNA.

Paper Details

Date Published: 25 January 2008
PDF: 8 pages
Proc. SPIE 6836, MEMS/MOEMS Technologies and Applications III, 68360O (25 January 2008); doi: 10.1117/12.769425
Show Author Affiliations
Hong Wang, South China Univ. of Technology (China)
Yu Tang, South China Univ. of Technology (China)

Published in SPIE Proceedings Vol. 6836:
MEMS/MOEMS Technologies and Applications III
Jung-Chih Chiao; Xuyuan Chen; Zhaoying Zhou; Xinxin Li, Editor(s)

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