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Proceedings Paper

Large field of view scanning fluorescence lifetime imaging system for multimode optical imaging of small animals
Author(s): Jae Youn Hwang; Hasmik Agadjanian; Lali K. Medina-Kauwe; Zeev Gross; Harry B. Gray; Karn Sorasaenee; Daniel L. Farkas
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Paper Abstract

We describe a scanning fluorescence lifetime imaging (SFLIM) system that provides a large field of view (LFOV), using a femtosecond (fs) pulsed laser, for multi-mode optical imaging of small animals. Fluorescence lifetime imaging (FLIM) can be a useful optical method to distinguish between fluorophores inside small animals. However, difficulty arises when LFOV is required in FLIM using a fs pulsed laser for the excitation of the fluorophores at low wavelengths (<500nm), primarily because the field of view of the pulsed blue excitation light generated from the second harmonic of the fs pulsed light is limited to about a centimeter in diameter due to the severe scattering and absorption of the light inside tissues. Here, we choose a scanning method in order to acquire a FLIM image with LFOV as one alternative. In the SFLIM system, we used a conventional cooled CCD camera coupled to an ultra-fast time-gated intensifier, a tunable femtosecond laser for the excitation of fluorophores, and an x-y moving stage for scanning. Images acquired through scanning were combined into a single image and then this reconstructed image was compared with images obtained by spectral imaging. The resulting SFLIM system is promising as an alternative method for the FLIM imaging of small animals, containing fluorophores exited by blue light, for LFOV applications such as whole animal imaging.

Paper Details

Date Published: 18 March 2008
PDF: 8 pages
Proc. SPIE 6859, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VI, 68590G (18 March 2008); doi: 10.1117/12.769305
Show Author Affiliations
Jae Youn Hwang, Univ. of Southern California (United States)
Cedars-Sinai Medical Ctr. (United States)
Hasmik Agadjanian, Cedars-Sinai Medical Ctr. (United States)
Lali K. Medina-Kauwe, Cedars-Sinai Medical Ctr. (United States)
Zeev Gross, Technion Israel Institute of Technology (Israel)
Harry B. Gray, California Institute of Technology (United States)
Karn Sorasaenee, Univ. of Southern California Keck School of Medicine (United States)
Daniel L. Farkas, Univ. of Southern California (United States)
Cedars-Sinai Medical Ctr. (United States)


Published in SPIE Proceedings Vol. 6859:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VI
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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