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Proceedings Paper

Glycoprotein expression by adenomatous polyps of the colon
Author(s): Celeste A. Roney; Jianwu Xie; Biying Xu; Paul Jabour; Gary Griffiths; Ronald M. Summers
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Paper Abstract

Colon cancer is the second leading cause of cancer related deaths in the United States. Specificity in diagnostic imaging for detecting colorectal adenomas, which have a propensity towards malignancy, is desired. Adenomatous polyp specimens of the colon were obtained from the mouse model of colorectal cancer called adenomatous polyposis coli-multiple intestinal neoplasia (APCMin). Histological evaluation, by the legume protein Ulex europaeus agglutinin I (UEA-1), determined expression of the glycoprotein α-L-fucose. FITC-labelled UEA-1 confirmed overexpression of the glycoprotein by the polyps on fluorescence microscopy in 17/17 cases, of which 13/17 included paraffin-fixed mouse polyp specimens. In addition, FITC-UEA-1 ex vivo multispectral optical imaging of 4/17 colonic specimens displayed over-expression of the glycoprotein by the polyps, as compared to non-neoplastic mucosa. Here, we report the surface expression of α-L-fucosyl terminal residues by neoplastic mucosal cells of APC specimens of the mouse. Glycoprotein expression was validated by the carbohydrate binding protein UEA-1. Future applications of this method are the development of agents used to diagnose cancers by biomedical imaging modalities, including computed tomographic colonography (CTC). UEA-1 targeting to colonic adenomas may provide a new avenue for the diagnosis of colorectal carcinoma by CT imaging.

Paper Details

Date Published: 12 March 2008
PDF: 10 pages
Proc. SPIE 6916, Medical Imaging 2008: Physiology, Function, and Structure from Medical Images, 69161O (12 March 2008); doi: 10.1117/12.769224
Show Author Affiliations
Celeste A. Roney, National Institutes of Health (United States)
Jianwu Xie, National Institutes of Health (United States)
Biying Xu, National Institutes of Health (United States)
National Heart, Lung, and Blood Institute (United States)
Paul Jabour, National Institutes of Health (United States)
Gary Griffiths, National Institutes of Health (United States)
National Heart, Lung, and Blood Institute (United States)
Ronald M. Summers, National Institutes of Health (United States)


Published in SPIE Proceedings Vol. 6916:
Medical Imaging 2008: Physiology, Function, and Structure from Medical Images
Xiaoping P. Hu; Anne V. Clough, Editor(s)

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