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Proceedings Paper

Combining AFM and FRET for studies at the cellular level
Author(s): Kenith E. Meissner; Zhe Sun; Bhavik Nathwani; Clark Needham; Richard E. Beckham; William N. Everett; Xudong Fan; Gerard L. Cote; Gerald A. Meininger
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Paper Abstract

We report on the development of a Forster resonance energy transfer (FRET) enabled atomic force microscope (AFM) for the study of biomechanics and mechanobiology at the cellular level. The hybrid microscopy system combines the spatial resolution and control of the AFM with the nanoscale sensing capabilities of FRET to enable simultaneous detection of cell mechanical responses and correlation of those responses with cellular biochemistry. Here, we show FRET signal from donor-coated microspheres, that are attached to an AFM cantilever, to acceptor-labeled integrins in a fixed cell system. Additionally, we demonstrate and discuss the attachment of quantum dots to silica microspheres as the FRET donor.

Paper Details

Date Published: 22 February 2008
PDF: 9 pages
Proc. SPIE 6863, Optical Diagnostics and Sensing VIII, 686302 (22 February 2008); doi: 10.1117/12.769175
Show Author Affiliations
Kenith E. Meissner, Texas A&M Univ. (United States)
Zhe Sun, Univ. of Missouri, Columbia (United States)
Bhavik Nathwani, Texas A&M Univ. (United States)
Clark Needham, Texas A&M Univ. (United States)
Richard E. Beckham, Texas A&M Univ. (United States)
William N. Everett, Texas A&M Univ. (United States)
Xudong Fan, Univ. of Missouri, Columbia (United States)
Gerard L. Cote, Texas A&M Univ. (United States)
Gerald A. Meininger, Univ. of Missouri, Columbia (United States)

Published in SPIE Proceedings Vol. 6863:
Optical Diagnostics and Sensing VIII
Gerard L. Coté; Alexander V. Priezzhev, Editor(s)

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